Q. What is
cloning?
Ans: In biological sense, a clone refers to cells with an
identical genotype. As used in recombinant DNA, a clone
specifically refers to identical host cells that carry identical recombinant
DNA molecule.
Q. What are various steps
involved in DNA cloning?
Ans: DNA cloning involves separating a specific gene or
segment of DNA from its larger chromosome and attacking it
to a small molecule of carrier DNA, then replicating this modified DNA
thousands or even millions of times. The result is a selective amplification of
that particular gene or DNA-segment.
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